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Anaes VivasMeasurement & monitoring physics

Anaes Vivas · Measurement & monitoring physics

Data viva — the oxyhaemoglobin dissociation curve

A data viva on the oxyhaemoglobin dissociation curve — the sigmoid shape, the flat top that makes SpO2 a late index of PaO2, the P50 at 3.5 kPa (26.8 mmHg), the left-shift factors (alkalosis, hypothermia, low 2,3-DPG) and the right-shift factors (acidosis, hyperthermia, high 2,3-DPG), and how the pulse oximeter's two wavelengths map onto it.

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Prompt
A graph of the oxyhaemoglobin dissociation curve. Saturation (per cent) is on the y-axis from 0 to 100; PaO2 (kPa) is on the x-axis from 0 to 16. The curve is sigmoid — flat near the top above about 8 kPa and steep below. A vertical dashed line marks the P50 at 3.5 kPa where the curve crosses 50 per cent saturation. A second curve is drawn shifted up and to the LEFT, labelled alkalosis, hypothermia, low 2,3-DPG. A third curve is drawn shifted down and to the RIGHT, labelled acidosis, hyperthermia, high 2,3-DPG. An inset shows two arrows for the pulse oximeter at 660 nm (red) and 940 nm (infrared).

Stimulus

You are shown the oxyhaemoglobin dissociation curve, with saturation on the y-axis against PaO2 in kPa on the x-axis. The curve is sigmoid. A dashed line marks the P50 at 3.5 kPa. Two further curves show a left shift (labelled alkalosis, hypothermia, low 2,3-DPG) and a right shift (labelled acidosis, hyperthermia, high 2,3-DPG). An inset shows the two wavelengths of the pulse oximeter at 660 nm and 940 nm. Interpret the graph.

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References3ShowHide
  1. [1]Moon K, et al. Pulse oximetry: a perioperative perspective. Diagnostics (Basel), 2026.PMID 42351472
  2. [3]Atuar B, et al. Reduce hypoxemia overestimation in pulse oximetry based on iso-pathlength: a Monte Carlo study. Opt Lett, 2026.PMID 42066129
  3. [7]Shi Y, et al. Diffusion correction of Beer-Lambert law in visible light optical coherence tomography for retinal vessels. Med Biol Eng Comput, 2026.PMID 42008039
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