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Derm Vivas

Derm Vivas ·

Special stains and immunofluorescence in dermatopathology — Viva

clinical1 min readVerification in progress
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Q1: Specimen handling (2 min)

Contrast formalin versus Michel medium. What is the correct DIF biopsy site?

Q2: DIF patterns (3 min)

Draw/describe fishnet IgG, linear BMZ IgG/C3, and granular IgA papillary patterns with one disease each.

Q3: Salt-split (2 min)

Explain the lamina lucida split and roof versus floor teaching rule.

Q4: Special stains menu (3 min)

When do you order PAS/GMS, AFB/Fite, VVG, and Congo red?

Q5: Pitfalls (2 min)

List three causes of false-negative DIF and one reason negative fungal stains may still not exclude infection.

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